| Analysis Name | Prunus mongolica ASM3034539v1 Assembly & Annotation |
| Sequencing technology | PacBio Sequel; Hi-C |
| Assembly method | Hifiasm v. 0.16; LACHESIS v. 0 |
| Release Date | 2023-06-27 |
Zhu Q, Wang Y, Yao N, Ni X, Wang C, Wang M, Zhang L, Liang W. Chromosome-level genome assembly of an endangered plant Prunus mongolica using PacBio and Hi-C technologies. DNA Res. 2023 Aug 1;30(4):dsad012. doi: 10.1093/dnares/dsad012.
AbstractPrunus mongolica is an ecologically and economically important xerophytic tree native to Northwest China. Here, we report a high-quality, chromosome-level P. mongolica genome assembly integrating PacBio high-fidelity sequencing and Hi-C technology. The assembled genome was 233.17 Mb in size, with 98.89% assigned to eight pseudochromosomes. The genome had contig and scaffold N50s of 24.33 Mb and 26.54 Mb, respectively, a BUSCO completeness score of 98.76%, and CEGMA indicated that 98.47% of the assembled genome was reliably annotated. The genome contained a total of 88.54 Mb (37.97%) of repetitive sequences and 23,798 protein-coding genes. We found that P. mongolica experienced two whole-genome duplications, with the most recent event occurring ~3.57 million years ago. Phylogenetic and chromosome syntenic analyses revealed that P. mongolica was closely related to P. persica and P. dulcis. Furthermore, we identified a number of candidate genes involved in drought tolerance and fatty acid biosynthesis. These candidate genes are likely to prove useful in studies of drought tolerance and fatty acid biosynthesis in P. mongolica, and will provide important genetic resources for molecular breeding and improvement experiments in Prunus species. This high-quality reference genome will also accelerate the study of the adaptation of xerophytic plants to drought.
Assembly statistics
| Genome size | 233.1 Mb |
| Total ungapped length | 233.1 Mb |
| Number of chromosomes | 8 |
| Number of scaffolds | 82 |
| Scaffold N50 | 26.5 Mb |
| Scaffold L50 | 4 |
| Number of contigs | 89 |
| Contig N50 | 24.3 Mb |
| Contig L50 | 5 |
| GC percent | 38 |
| Genome coverage | 42.0x |
| Assembly level | Chromosome |
The Prunus mongolica ASM3034539v1 Assembly file is available in FASTA format.
Downloads
| Chromosomes (FASTA file) | GCA_030345395.1_ASM3034539v1_genomic.fna.gz |
The Prunus mongolica ASM3034539v1 genome gene prediction files are available in GFF3 and FASTA format.
Downloads
| Genes (GFF3 file) | genome.gff3.gz |
| CDS sequences (FASTA file) | cds.gff3.cds.gz |
| Protein sequences (FASTA file) | protein.gff3.pep.gz |
Functional annotation for the Prunus mongolica ASM3034539v1 is available for download below. The proteins were analyzed using InterProScan to assign InterPro domains(Pfam).
Downloads
| Domain from InterProScan | Prunus_mongolica_Whole_Genome_v1.0.Pfam.tsv.gz |
Prunus S genes Nucleotide
Prunus S genes Protein