Prunus humilis Genome v1.0 Assembly & Annotation

Overview

Analysis Name Prunus humilis Genome v1.0 Assembly & Annotation
Sequencing technology Illumina, Pacbio and HiC reads
Assembly method CANU (1.8)
Release Date 2022-07-11
Reference Publication(s)

Wang Y, Xie J, Zhang H, Li W, Wang Z, Li H, Tong Q, Qiao G, Liu Y, Tian Y, Wei Y, Li P, Wang R, Chen W, Liang Z, Xu M. The genome of Prunus humilis provides new insights to drought adaption and population diversity. DNA Res. 2022 Jun 25;29(4):dsac021. doi: 10.1093/dnares/dsac021.

Abstract

Prunus humilis (2n = 2x = 16) is a dwarf shrub fruit tree native to China and distributed widely in the cold and arid northern region. In this study, we obtained the whole genome sequences of P. humilis by combining Illumina, PacBio and HiC sequencing technologies. This genome was 254.38 Mb long and encodes 28,301 putative proteins. Phylogenetic analysis indicated that P. humilis shares the same ancestor with Prunus mume and Prunus armeniaca at ∼ 29.03 Mya. Gene expansion analysis implied that the expansion of WAX-related and LEA genes might be associated with high drought tolerance of P. humilis and LTR maybe one of the driver factors for the drought adaption by increase the copy number of LEAs. Population diversity analysis among 20 P. humilis accessions found that the genetic diversity of P. humilis populations was limited, only 1.40% base pairs were different with each other, and more wild resources need to be collected and utilized in the breeding and improvement. This study provides new insights to the drought adaption and population diversity of P. humilis that could be used as a potential model plant for horticultural research.

Assembly

The Prunus humilis Genome v1.0 Assembly file is available in FASTA format.

Downloads

Chromosomes (FASTA file) ol.fasta.gz

Gene Predictions

The Prunus humilis Genome v1.0 genome gene prediction files are available in GFF3 and FASTA format.

Downloads

Genes (GFF3 file) ol.gff3.gz
CDS sequences (FASTA file) ol.cds.gz
Protein sequences (FASTA file) ol.prot.gz

Functional Analysis

Functional annotation for the Prunus humilis Genome v1.0 is available for download below. The proteins were analyzed using InterProScan to assign InterPro domains(Pfam).

Downloads

Domain from InterProScan Prunus_humilis_Genome_v1.0.Pfam.tsv.gz

S genes

Prunus S genes Nucleotide

Prunus S genes Protein

© 2023 National Genomics Data Center, China National Center for Bioinformation / Beijing Institute of Genomics, Chinese Academy of Sciences