| Analysis Name | Prunus avium Tieton Genome v2.0 Assembly & Annotation |
| Sequencing technology | Oxford Nanopore PromethION; Illumina HiSeq |
| Assembly method | Necat v. 0.01 |
| Release Date | 2020-08-13 |
Wang J, Liu W, Zhu D, Hong P, Zhang S, Xiao S, Tan Y, Chen X, Xu L, Zong X, Zhang L, Wei H, Yuan X, Liu Q. Chromosome-scale genome assembly of sweet cherry (Prunus avium L.) cv. Tieton obtained using long-read and Hi-C sequencing. Hortic Res. 2020 Aug 1;7(1):122. doi: 10.1038/s41438-020-00343-8.
AbstractSweet cherry (Prunus avium) is an economically significant fruit species in the genus Prunus. However, in contrast to other important fruit trees in this genus, only one draft genome assembly is available for sweet cherry, which was assembled using only Illumina short-read sequences. The incompleteness and low quality of the current sweet cherry draft genome limit its use in genetic and genomic studies. A high-quality chromosome-scale sweet cherry reference genome assembly is therefore needed. A total of 65.05 Gb of Oxford Nanopore long reads and 46.24 Gb of Illumina short reads were generated, representing ~190x and 136x coverage, respectively, of the sweet cherry genome. The final de novo assembly resulted in a phased haplotype assembly of 344.29 Mb with a contig N50 of 3.25 Mb. Hi-C scaffolding of the genome resulted in eight pseudochromosomes containing 99.59% of the bases in the assembled genome. Genome annotation revealed that more than half of the genome (59.40%) was composed of repetitive sequences, and 40,338 protein-coding genes were predicted, 75.40% of which were functionally annotated. With the chromosome-scale assembly, we revealed that gene duplication events contributed to the expansion of gene families for salicylic acid/jasmonic acid carboxyl methyltransferase and ankyrin repeat-containing proteins in the genome of sweet cherry. Four auxin-responsive genes (two GH3s and two SAURs) were induced in the late stage of fruit development, indicating that auxin is crucial for the sweet cherry ripening process. In addition, 772 resistance genes were identified and functionally predicted in the sweet cherry genome. The high-quality genome assembly of sweet cherry obtained in this study will provide valuable genomic resources for sweet cherry improvement and molecular breeding.
Assembly statistics
| Genome size | 344.3 Mb |
| Total ungapped length | 344.3 Mb |
| Number of chromosomes | 8 |
| Number of scaffolds | 61 |
| Scaffold N50 | 42.6 Mb |
| Scaffold L50 | 4 |
| Number of contigs | 610 |
| Contig N50 | 3.2 Mb |
| Contig L50 | 32 |
| GC percent | 38.5 |
| Genome coverage | 1.0x |
| Assembly level | Chromosome |
The Prunus avium Tieton Genome v2.0 Assembly file is available in FASTA format.
Downloads
| Chromosomes (FASTA file) | Prunus_avium_Tieton.chr.fasta.gz |
The Prunus avium Tieton Genome v2.0 genome gene prediction files are available in GFF3 and FASTA format.
Downloads
| Genes (GFF3 file) | Prunus_avium_Tieton.annotation.gff3.gz |
| CDS sequences (FASTA file) | Prunus_avium_Tieton.cds-transcripts.fasta.gz |
| Protein sequences (FASTA file) | Prunus_avium_Tieton.proteins.fa.gz |
Functional annotation for the Prunus avium Tieton Genome v2.0 is available for download below. The proteins were analyzed using InterProScan to assign InterPro domains(Pfam).
Downloads
| Domain from InterProScan | Prunus_avium_Tieton_Genome_v2.0.Pfam.tsv.gz |
Summary
| Query | Chr | Size(bp) | Coordinates | BLASTn Hit | BLASTn %ID | Domain |
| SLF7.2 | chr_6 | 42624765 | 34013988-34015454 | ParSLF7.2 | 94.558 | F-box; F_box_assoc |
| SLF7.1 | chr_6 | 42624765 | 34459914-34461386 | ParSLF7.1 | 93.854 | F-box |
| SLF4 | chr_6 | 42624765 | 34904593-34903235 | ParSLF4 | 97.149 | F-box; F_box_assoc |
| SLF2 | chr_6 | 42624765 | 34992157-34993431 | ParSLF2 | 97.743 | F-box; F_box_assoc |
| SFB | chr_6 | 42624765 | 35028431-35029561 | ParSFB | 87.456 | F-box; F_box_assoc |
| SLF1 | chr_6 | 42624765 | 35108882-35107650 | ParSLF1 | 90.96 | F-box; F_box_assoc |
| SLF3 | chr_6 | 42624765 | 35143581-35144831 | ParSLF3 | 96.729 | F-box; F_box_assoc |
| SLF5 | chr_6 | 42624765 | 35183943-35182765 | ParSLF5 | 98.134 | F-box; F_box_assoc |
| SLF6 | chr_6 | 42624765 | 36517543-36516344 | ParSLF6 | 98.917 | F-box |
| S-RNase | chr_6 | 42624765 | 35093454-35093376,35093105-35092921,35092527-35092117 | AJ298310.1, RNase S1 | 90.8 | RNase_T2 |
Prunus avium Tieton_Genome_v2.0 S genes Nucleotide
Prunus avium Tieton_Genome_v2.0 S genes Protein